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KMID : 0370219960400060625
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Yakhak Hoeji
1996 Volume.40 No. 6 p.625 ~ p.631
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Characteristic Responses of Biosensor Based on H+-Selective Membrane Electrode
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김기묘/Kim KM
박성배/황명석/허문회/안문규/Park SB/Hwang MS/Hur MH/Ahn MK
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Abstract
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The H+-selective membrane consists of tridodecylamine, 2-nitrophenyl octyl ether and a little amount of additive in carboxylated PVC matrix, where penicillinase(Pcase) and glutaraldehyde may be covalently attached to the matrix. When the H+-selective electrode was used as a detector of biosensor, calibration curve calculated from Nernst equation was not linear. So we investigated the characteristic responses of the H+-selective electrode to the product H+ from hydrolysis of penicillin-G(Pc-G) and plotted calibration curve correlating potential to concentration of Pc-G linearly. The optimal concentration of buffer solution was theoretically calculated and was also experimented. We tried to explain the linear curve of potential to concentration of Pc-G by using Henderson-Hasselbach equation. This method is more effective in calibration curve plotting than any other previous methods. The results obtained may help in further developing pH electrodes with improved analytical preformance.
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